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Hematopoietic stem cell (HSC) transplantation is a rapidly evolving technique that offers a potential cure for hematologic cancers (leukemias, lymphomas, myeloma) and other hematologic disorders (eg, primary immunodeficiency, aplastic anemia, myelodysplasia). HSC transplantation may be autologous or allogeneic; bone marrow, peripheral blood, or umbilical cord stem cells may be used. Peripheral blood has largely replaced bone marrow as a source of stem cells, especially in autologous HSC transplantation, because stem cell harvest is easier and neutrophil and platelet counts recover faster. Umbilical cord HSC transplantation has been restricted mainly to children because the number of stem cells is low.
There are no contraindications to autologous HSC transplantation. Contraindications to allogeneic HSC transplantation are relative and include age > 50, previous HSC transplantation, and significant comorbidities. Allogeneic HSC transplantation is limited mainly by lack of histocompatible donors. An HLA-identical sibling donor is ideal, followed by an HLA-matched sibling donor. Because only one fourth of patients have such a sibling donor, mismatched related or matched unrelated donors (identified through international registries) are often used. However, long-term disease-free survival rates may be lower than those with HLA-identical sibling donors. The technique for umbilical cord HSC transplantation is still being defined, but HLA-matching is probably unimportant.
Procedure
For bone marrow stem cell harvest, 700 to 1500 mL (maximum 15 mL/kg) of marrow is aspirated from the donor's posterior iliac crests; local or general anesthesia is used. For peripheral blood harvest, the donor is treated with recombinant growth factors (granulocyte colony-stimulating factor or granulocyte-macrophage colony-stimulating factor) to stimulate proliferation and mobilization of stem cells, with standard phlebotomy 4 to 6 days afterward. Fluorescence-activated cell sorting is used to identify and separate stem cells from other cells.
Stem cells are then infused over 1 to 2 h through a large-bore central venous catheter. In HSC transplantation for cancer, the recipient first is given a conditioning regimen (eg, cyclophosphamide 60 mg/kg IV once/day for 2 days with total body irradiation, busulfan 1 mg/kg po qid for 4 days plus cyclophosphamide without total body irradiation) to induce remission and suppress the immune system so that the graft can be accepted. Similar regimens are used for allogeneic HSC transplantation, even when cancer is not the indication, to reduce incidence of rejection and relapse, but not for autologous HSC transplantation. Nonmyeloablative conditioning regimens may reduce morbidity and mortality risks and may be useful for elderly patients, patients with comorbidities, and patients susceptible to a graft-vs-tumor effect (eg, those with multiple myeloma).
After transplantation, recipients are given colony-stimulating factors to shorten duration of posttransplantation leukopenia, prophylactic anti-infective drugs (see Transplantation: Infection), and, in allogeneic HSC transplantation, up to 6 mo of prophylactic immunosuppressants (typically methotrexate and cyclosporine) to prevent donor T cells from reacting against recipient major histocompatibility complex molecules (graft-vs-host disease [GVHD]). Broad-spectrum antibiotics are usually withheld unless fever develops. Engraftment typically occurs 10 to 20 days after HSC transplantation (earlier with peripheral blood stem cells) and is defined by an absolute neutrophil count > 500 × 106/L.
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Major early complications (< 100 days) include
Failure to engraft and rejection affect < 5% of patients and manifest as persistent pancytopenia or irreversible decline in blood counts. Treatment is corticosteroids for several weeks.
Acute GVHD occurs in recipients of allogeneic HSC transplants, 40% of HLA-matched sibling graft recipients, and 80% of unrelated donor graft recipients. It causes fever, rash, hepatitis with hyperbilirubinemia, vomiting, diarrhea, abdominal pain (which may progress to ileus), and weight loss. Risk factors include HLA and sex mismatching; unrelated donor; older age of recipient, donor, or both; donor presensitization; and inadequate GVHD prophylaxis. Diagnosis is obvious by history and physical examination; treatment is methylprednisolone 2 mg/kg IV once/day, increased to 10 mg/kg if there is no response within 5 days.
Major later complications include
Chronic GVHD may occur by itself, develop from acute GVHD, or occur after resolution of acute GVHD. It typically occurs 4 to 7 mo after HSC transplantation (range 2 mo to 2 yr). Chronic GVHD occurs in recipients of allogeneic HSC transplants, about 35 to 50% of HLA-matched sibling graft recipients, and 60 to 70% of unrelated donor graft recipients. It affects primarily the skin (eg, lichenoid rash, scleroderma) and mucous membranes (eg, keratoconjunctivitis sicca, periodontitis, orogenital lichenoid reactions), but it also affects the GI tract and liver. Immunodeficiency is a primary feature; bronchiolitis obliterans similar to that after lung transplantation can also develop. Ultimately, 20 to 40% die of GVHD; mortality rate is higher with more severe reactions. Treatment may not be necessary for skin and mucous membrane disease; treatment of more extensive disease is similar to that of acute GVHD. T-cell depletion of allogeneic donor grafts using monoclonal antibodies or mechanical separation reduces incidence and severity of GVHD but also eliminates a graft-vs-tumor effect that may enhance stem cell proliferation and engraftment and reduce disease relapse rates. Relapse rates with autologous HSC transplantation are higher for this reason and because circulating tumor cells may be transplanted. Ex vivo tumor cell purging before autologous transplantation is under study.
In patients without chronic GVHD, all immunosuppression can be stopped 6 mo after HSC transplantation; thus, late complications are rare in these patients.
Prognosis
Prognosis varies by indication and procedure. Overall, disease relapse occurs in 40 to 75% of recipients of autologous HSC transplants and in 10 to 40% of recipients of allogeneic HSC transplants. Success (cancer-free bone marrow) rates are 30 to 40% for patients with relapsed, chemotherapy-sensitive lymphoma and 20 to 50% for patients with acute leukemia in remission; compared with chemotherapy alone, HSC transplantation improves survival of patients with multiple myeloma. Success rates are low for patients with more advanced disease or with responsive solid cancers (eg, breast cancer, or germ cell tumors). Relapse rates are reduced in patients with GVHD, but overall mortality rates are increased if GVHD is severe. Intensive preparative regimens, effective GVHD prophylaxis, cyclosporine-based regimens, and improved supportive care (eg, antibiotics, herpesvirus and cytomegalovirus prophylaxis) have increased long-term disease-free survival after HSC transplantation.
Last full review/revision September 2008 by Martin Hertl, MD, PhD; James F. Markmann, MD, PhD; Paul S. Russell, MD; Heidi Yeh, MD
Content last modified September 2008
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