Nucleic acid–based methods detect organism-specific DNA or RNA sequences extracted from the microorganism. Sequences may or may not be amplified in vitro.
Nucleic acid–based (molecular) identification has become commonplace in clinical settings; the resulting rapid identification allows the patient to be placed on specific antimicrobial therapy and avoid prolonged management on empiric, potentially inappropriate drugs.
Nucleic acid–based methods are generally specific and highly sensitive and can be used for all categories of microbes. Results can be provided rapidly. Because each test typically is specific to a single organism, the clinician must know the diagnostic possibilities and request tests accordingly. For example, if a patient has symptoms suggesting influenza but the influenza season is over, doing a more general viral diagnostic test (eg, viral culture) rather than a specific flu test is better because another virus (eg, parainfluenza, adenovirus) may be the cause.
Recent advances have led to the development of multiplex assays, in which a single nucleic acid–based test can detect and differentiate between ≥ 2 causative microorganisms. Multiplex assays are currently available for detecting biological warfare agents, SARS-CoV-2 variants, and certain respiratory tract pathogens (ie, multiplex assays encompassing a panel of respiratory viruses such RSV [respiratory syncytial virus], adenovirus, influenza, parainfluenza, as well as non-viral pathogens such as pneumococci, mycoplasmas, and chlamydiae). Multiplex assays have similar sensitivity and specificity as single target assays but are mostly qualitative and may be more difficult to interpret in a given patient.
Nucleic acid–based tests are qualitative, but quantification methods exist for a limited but increasing number of infections (eg, hepatitis B Hepatitis B, Acute Hepatitis B is caused by a DNA virus that is often parenterally transmitted. It causes typical symptoms of viral hepatitis, including anorexia, malaise, and jaundice. Fulminant hepatitis and... read more , hepatitis C Hepatitis C, Acute Hepatitis C is caused by an RNA virus that is often parenterally transmitted. It sometimes causes typical symptoms of viral hepatitis, including anorexia, malaise, and jaundice but may be asymptomatic... read more , HIV Diagnosis Human immunodeficiency virus (HIV) infection results from 1 of 2 similar retroviruses (HIV-1 and HIV-2) that destroy CD4+ lymphocytes and impair cell-mediated immunity, increasing risk of certain... read more , cytomegalovirus Diagnosis Cytomegalovirus (CMV, human herpesvirus type 5) can cause infections that have a wide range of severity. A syndrome of infectious mononucleosis that lacks severe pharyngitis is common. Severe... read more , human T-cell lymphotropic virus); these methods can be useful for diagnosis and for monitoring response to treatment.
Techniques that target nucleic acid sequences but do not require amplification of those sequences are usually restricted to situations in which the organism has been first cultured or is present in high concentration in the specimen (eg, in pharyngitis Testing Sore throat is pain in the posterior pharynx that occurs with or without swallowing. Pain can be severe; many patients refuse oral intake. Sore throat results from infection; the most common... read more caused by group A Streptococcus, in genital infections caused by Chlamydia trachomatis Chlamydia and Mycoplasmal Mucosal Infections Sexually transmitted urethritis, cervicitis, proctitis, and pharyngitis (that are not due to gonorrhea) are caused predominantly by chlamydiae and less frequently by mycoplasmas. Chlamydiae... read more or Neisseria gonorrhoeae Gonorrhea Gonorrhea is caused by the bacterium Neisseria gonorrhoeae. It typically infects epithelia of the urethra, cervix, rectum, pharynx, or conjunctivae, causing irritation or pain and purulent... read more ).
Nucleic acid amplification
Nucleic acid amplification techniques (NAAT) take tiny amounts of DNA or RNA, replicate them many times, and thus can detect minute traces of an organism in a specimen, avoiding the need for culture. These techniques are particularly useful for organisms that are difficult to culture or identify using other methods (eg, viruses, obligate intracellular pathogens, fungi, mycobacteria, some other bacteria) or that are present in low numbers.
These tests may involve
Target amplification (eg, polymerase chain reaction [PCR], reverse transcriptase–PCR [RT-PCR], strand displacement amplification, transcription amplification)
Signal amplification (eg, branched DNA assays, hybrid capture)
Probe amplification (eg, ligase chain reaction, cleavase-invader, cycling probes)
Postamplification analysis (eg, sequencing of the amplified product, microarray analysis, and melting curve analysis, as is done in real-time PCR)
Appropriate specimen collection and storage before arrival at the molecular diagnostic laboratory are critical. Because amplification methods are so sensitive, false-positive results from trace contamination of the specimen or equipment can easily occur.
Despite high sensitivity, false-negative results sometimes occur even when a patient is symptomatic (eg, in West Nile virus infection West Nile Virus West Nile virus is a flavivirus that is now the primary cause of arbovirus encephalitis in the United States. Most patients have mild or no symptoms. About 1 out of 150 patients develop a severe... read more ). False-negative results can be minimized by the following:
Avoiding use of swabs with wooden shafts or cotton tips (the swab that has been validated for the amplification assay must be used)
Transporting specimens rapidly
Freezing or refrigerating specimens if transport is likely to take > 2 hours
Freezing is the typical storage method for nucleic acid amplification assays. However, specimens should be refrigerated rather than frozen if labile viruses (eg, varicella-zoster virus, influenza virus, HIV-2) are suspected or if viral cultures are also to be done (frozen specimens may not be usable for standard cultures).