Merck Manual

Please confirm that you are a health care professional


Cushing Syndrome


Ashley B. Grossman

, MD, University of Oxford; Fellow, Green-Templeton College

Last full review/revision Jul 2019| Content last modified Jul 2019
Click here for Patient Education
NOTE: This is the Professional Version. CONSUMERS: Click here for the Consumer Version
Topic Resources

Cushing syndrome is a constellation of clinical abnormalities caused by chronic high blood levels of cortisol or related corticosteroids. Cushing disease is Cushing syndrome that results from excess pituitary production of adrenocorticotropic hormone (ACTH), generally secondary to a pituitary adenoma. Typical symptoms and signs include moon face and truncal obesity, easy bruising, and thin arms and legs. Diagnosis is by history of receiving corticosteroids or by finding elevated serum cortisol. Treatment depends on the cause.


Hyperfunction of the adrenal cortex can be adrenocorticotropic hormone (ACTH)-dependent or ACTH-independent.

ACTH-dependent hyperfunction may result from

ACTH-independent hyperfunction usually results from

  • Therapeutic administration of corticosteroids

  • Adrenal adenomas or carcinomas

Rare causes of ACTH-independent hyperfunction include primary pigmented nodular adrenal dysplasia (usually in adolescents) and macronodular dysplasia (in older patients).

Whereas the term Cushing syndrome denotes the clinical picture resulting from cortisol excess from any cause, Cushing disease refers to hyperfunction of the adrenal cortex from pituitary ACTH excess. Patients with Cushing disease usually have a small adenoma of the pituitary gland.

Symptoms and Signs

Clinical manifestations of Cushing syndrome include

  • Moon face with a plethoric appearance

  • Truncal obesity with prominent supraclavicular and dorsal cervical fat pads (buffalo hump)

  • Usually, very slender distal extremities and fingers

Manifestations of Cushing Syndrome

Muscle wasting and weakness are present. The skin is thin and atrophic, with poor wound healing and easy bruising. Purple striae may appear on the abdomen. Hypertension, renal calculi, osteoporosis, glucose intolerance, reduced resistance to infection, and mental disturbances are common. Cessation of linear growth is characteristic in children.

Females usually have menstrual irregularities. In females with adrenal tumors, increased production of androgens may lead to hirsutism, temporal balding, and other signs of virilism.


  • Urinary free cortisol (UFC) level

  • Dexamethasone suppression test

  • Midnight serum or salivary cortisol levels

  • Plasma ACTH levels; if detectable, provocative testing

Diagnosis is usually suspected based on the characteristic symptoms and signs. Confirmation (and identification of the cause) generally requires hormonal and imaging tests.

Urinary free cortisol measurement

In some centers, testing begins with measurement of UFC, the best assay for urinary excretion (normal, 20 to 100 mcg/24 hours [55.2 to 276 nmol/24 hours]). UFC is elevated > 120 mcg/24 hours (>331 nmol/24 hours) in almost all patients with Cushing syndrome. However, many patients with UFC elevations between 100 and 150 mcg/24 hours (276 and 414 nmol/24 hours) have obesity, depression, or polycystic ovaries but not Cushing syndrome.

A patient with suspected Cushing syndrome with grossly elevated UFC (> 4 times the upper limit of normal) almost certainly has Cushing syndrome. Two to three normal collections usually excludes the diagnosis. Slightly elevated levels generally necessitate further investigation, as do normal levels when clinical suspicion is high.

A baseline morning (eg, 9 AM) serum cortisol measurement should also be done.

Dexamethasone suppression test

An alternative approach to investigation uses the dexamethasone suppression test, in which 1, 1.5, or 2 mg of dexamethasone is given orally at 11 to 12 PM and serum cortisol is measured at 8 to 9 AM the next morning. In most normal patients, this drug suppresses morning serum cortisol to < 1.8 mcg/mL (< 50 nmol/L), whereas patients with Cushing syndrome virtually always have a higher level. A more specific but equally sensitive test is to give dexamethasone 0.5 mg orally every 6 hours for 2 days (low dose). In general, a clear failure to suppress cortisol levels in response to low-dose dexamethasone establishes the diagnosis.

Midnight cortisol measurements

If results of UFC measurements and the dexamethasone suppression test are indeterminate, the patient is hospitalized for measurement of serum cortisol at midnight, which is more likely to be conclusive. Alternatively, salivary cortisol samples may be collected and stored in the refrigerator at home. Serum cortisol normally ranges from 5 to 25 mcg/dL (138 to 690 nmol/L) in the early morning (6 to 8 AM) and declines gradually to < 1.8 mcg/dL (< 50 nmol/L) at midnight. Patients with Cushing syndrome occasionally have a normal morning serum cortisol level but lack normal diurnal decline in cortisol production, such that the midnight serum cortisol levels are above normal and the total 24-hour cortisol production may be elevated.

Serum cortisol may be spuriously elevated in patients with congenital increases of corticosteroid-binding globulin or in those receiving estrogen therapy, but diurnal variation is normal in these patients.

Plasma ACTH measurement

ACTH levels are measured to determine the cause of Cushing syndrome. Undetectable levels, both basally and particularly in response to corticotropin-releasing hormone (CRH), suggest a primary adrenal cause. High levels suggest a pituitary cause or an ectopic source. If ACTH is detectable, provocative tests help differentiate Cushing disease from ectopic ACTH syndrome, which is rarer. In response to high-dose dexamethasone (2 mg orally every 6 hours for 48 hours), the 9 AM serum cortisol falls by > 50% in most patients with Cushing disease but infrequently in those with ectopic ACTH syndrome. Conversely, ACTH rises by > 50% and cortisol rises by 20% in response to human or ovine-sequence CRH (100 mcg IV or 1 mcg/kg IV) in most patients with Cushing disease but very rarely in those with ectopic ACTH syndrome (see table Diagnostic Tests in Cushing Syndrome).

An alternative approach to localization, which is more accurate but more invasive, is to catheterize both petrosal veins (which drain the pituitary) and measure ACTH from these veins 5 minutes after giving a 100 mcg or 1 mcg/kg bolus of CRH (human or ovine). A central-to-peripheral ACTH ratio > 3 virtually excludes ectopic ACTH syndrome, whereas a ratio < 3 suggests a need to seek such a source.


Diagnostic Tests in Cushing Syndrome


Serum Cortisol, 9 AM

Salivary or Serum Cortisol, Midnight

Urinary Free Cortisol

Low-Dose or Overnight Dexamethasone Suppression Test

High-Dose Dexamethasone Suppression Test

Corticotropin-Releasing Hormone Testing

ACTH Level









Cushing disease

N or



N or

N or

Ectopic ACTH

N or




↑ or ↑↑

Adrenal tumor

N or




* May be elevated in non-Cushing conditions.

ACTH = adrenocorticotropic hormone; Flat = no significant rise in ACTH or cortisol; N = normal; NS = nonsuppression; S = suppression; ↑↑= greatly increased; = increased; = decreased.


Pituitary imaging is done if ACTH levels and provocative tests suggest a pituitary cause; gadolinium-enhanced MRI is most accurate, but some microadenomas are visible on CT. If testing suggests a nonpituitary cause, imaging includes high-resolution CT of the chest, pancreas, and adrenals; scintiscanning or PET scanning with radiolabeled octreotide or, preferably, gallium-68 dotatate and occasionally fluorodeoxyglucose (FDG)-PET scanning. Petrosal sinus sampling may be needed to differentiate pituitary from ectopic sources.

In children with Cushing disease, pituitary tumors are very small and usually cannot be detected with MRI. Petrosal sinus sampling is particularly useful in this situation. MRI is preferred to CT in pregnant women to avoid fetal exposure to radiation.


  • High protein intake, and potassium administration (or potassium-sparing drugs such as spironolactone)

  • Adrenal inhibitors such as metyrapone, mitotane, or ketoconazole

  • Surgery or radiation therapy to remove pituitary, adrenal, or ectopic ACTH-producing tumors

  • Sometimes somatostatin analogs, dopamine agonists, or mifepristone

Initially, the patient’s general condition should be supported by high protein intake and appropriate administration of potassium. If clinical manifestations are severe, it may be reasonable to block corticosteroid secretion with metyrapone 250 mg to 1 g orally 3 times a day or ketoconazole 400 mg orally once a day, increasing to a maximum of 400 mg 3 times a day. Ketoconazole is probably slower in onset and sometimes hepatotoxic. Parenteral etomidate (an intravenous anesthetic that also blocks cortisol production) may be life-saving for patients with fulminant symptoms; it is given as an intravenous infusion: the starting dose is usually 1 to 2 mg/hour, increasing as necessary, with frequent assessments of cortisol levels and the dose titrated accordingly .

Pituitary tumors that produce excessive ACTH are removed surgically or extirpated with radiation therapy. If no tumor is shown on imaging but a pituitary source is likely, total hypophysectomy may be attempted, particularly in older patients. Younger patients usually receive supervoltage irradiation of the pituitary, delivering 45 Gy (Gray). However, in children, irradiation may reduce secretion of growth hormone and occasionally cause precocious puberty. In special centers, heavy particle beam irradiation, providing about 100 Gy, is often successful, as is a single focused beam of radiation therapy given as a single dose (radiosurgery). Response to irradiation occasionally requires several years, but response is more rapid in children.

Studies suggest that mild cases of persistent or recurrent disease may benefit from the somatostatin analog pasireotide. However, hyperglycemia is a significant adverse effect. The dopamine agonist cabergoline may also occasionally be useful. Alternatively, the corticosteroid receptors can be blocked with mifepristone. Mifepristone increases serum cortisol but blocks the effects of the corticosteroid and may cause hypokalemia.

Bilateral adrenalectomy is reserved for patients with pituitary hyperadrenocorticism who do not respond to both pituitary exploration (with possible adenomectomy) and irradiation, or in patients in whom surgery was unsuccessful and radiotherapy is contraindicated. Adrenalectomy requires life-long corticosteroid replacement.

Adrenocortical tumors are removed surgically. Patients must receive cortisol during the surgical and postoperative periods because their nontumorous adrenal cortex will be atrophic and suppressed. Benign adenomas can be removed laparoscopically. With multinodular adrenal hyperplasia, bilateral adrenalectomy may be necessary. Even after a presumed total adrenalectomy, functional regrowth occurs in a few patients.

Ectopic ACTH syndrome is treated by removing the nonpituitary tumor that is producing the ACTH. However, in some cases, the tumor is disseminated and cannot be excised. Adrenal inhibitors, such as metyrapone 500 mg orally 3 times a day (and up to a total of 6 g a day) or mitotane 0.5 g orally once a day, increasing to a maximum of 3 to 4 g a day, usually control severe metabolic disturbances (eg, hypokalemia). When mitotane is used, large doses of hydrocortisone or dexamethasone may be needed. Measures of cortisol production may be unreliable, and severe hypercholesterolemia may develop. Ketoconazole 400 to 1200 mg orally once a day also blocks corticosteroid synthesis, although it may cause liver toxicity and can cause addisonian symptoms. Mifepristone also may be useful for treating ectopic ACTH syndrome.

Sometimes ACTH-secreting tumors respond to long-acting somatostatin analogs, although administration for > 2 years requires close follow-up because mild gastritis, gallstones, cholangitis, and malabsorption may develop.

Nelson syndrome

Nelson syndrome occurs when the pituitary gland continues to expand after bilateral adrenalectomy, causing a marked increase in the secretion of ACTH and its precursors and resulting in severe hyperpigmentation. It occurs in 50% of patients who undergo adrenalectomy. The risk is probably reduced if the patient undergoes pituitary radiation therapy.

Although irradiation may arrest continued pituitary growth, many patients also require hypophysectomy. The indications for hypophysectomy are the same as for any pituitary tumor: an increase in size such that the tumor encroaches on surrounding structures, causing visual field defects, pressure on the hypothalamus, or other complications.

Routine irradiation is often done after hypophysectomy if it was not done previously, especially when a tumor is clearly present. Radiation therapy may be delayed if there is no obvious lesion. Radiosurgery, or focused radiation therapy, can be given in a single fraction when standard external beam radiation therapy has already been done, as long as the lesion is at a reasonable distance from the optic nerve and chiasm.

Key Points

  • Diagnosis is usually made by elevated nocturnal serum or salivary cortisol levels, or 24-hour urinary free cortisol level, and a dexamethasone suppression test in which serum cortisol does not suppress.

  • Pituitary causes are distinguished from nonpituitary causes by adrenocorticotropic hormone (ACTH) levels.

  • Imaging is then done to identify any causative tumor.

  • Tumors are usually treated surgically or with radiation therapy.

  • Metyrapone or ketoconazole may be given to suppress cortisol secretion prior to definitive treatment.

Drugs Mentioned In This Article

Drug Name Select Trade
No US brand name
Click here for Patient Education
NOTE: This is the Professional Version. CONSUMERS: Click here for the Consumer Version
Professionals also read

Also of Interest


View All
Overview of the Syndrome of Inappropriate Antidiuretic...
Overview of the Syndrome of Inappropriate Antidiuretic...
3D Models
View All
Female Endocrine System
3D Model
Female Endocrine System